KATHRYN J. BRAYER
124 Ashley Circle #4
Thesis: Analysis of DNA-Protein Interactions on Non-Functional Replication Origins of Escherichia coli Chromosomes.
Job responsibilities: assist in the development and validation of novel mutation detection systems using transgenic fish; refine protocols for exposing transgenic fish to known and suspected carcinogens/mutagens and anti-cancer agents; recover target DNA (transgene) for mutation analysis including DNA sequencing to determine location and type of DNA damage; screen all transgenic lines for presence of transgene, determination of homozygous/hemizygous status and of transgene copy number using standard PCR and quantitative PCR; develop and maintain database of mutation analysis results; maintain breeding population of transgenic fish; supervise and train graduate students; sustain lab operations; assist with grant proposals and journal/conference preparation; order lab supplies.
Conducted a literature search on population viability analysis.
Fed hatchery salmon, assisted during spawning, cleaned tanks.
Collected samples and set up displays on educational cruises for high school students.
Designed, set-up and broke-down experiments; collected and analyzed data; supervised high school volunteers, cared for experimental animals, maintained lab.
Analysis of oriC DNA-protein interactions under conditions where unwinding is prohibited. in American Society for Microbiology 98th General Meeting, Atlanta, GA, May 17-21, 1998
Analysis of oriC DNA-protein interactions under conditions where unwinding is prohibited. in Florida Branch of the American Society for Microbiology Meeting, Indialantic, FL, Feb. 27-28, 1998
Evaluation of transgenic fish for detecting in vivo mutations. Abstracts of the Pollutant Responses in Marine Organisms 10th International Symposium, Williamsburg, VA, April 25-29, 1999.
Analysis of oriC DNA-protein interactions under conditions where unwinding is prohibited. Abstracts of the Florida Branch of the American Society for Microbiology Meeting, Indialantic, FL, Feb. 27-28, 1998
Shelter use by juvenile American lobsters, Homarus americanus, under predation risk. in Abstracts of the 21st Marine Benthic Ecology Meetings, Mystic, CT, March 17-20, 1994.
Use of shelter by juvenile lobsters, Homarus americanus, under predation risk: responses of naive (laboratory) versus experienced (wild) lobsters. Abstracts of the Fourth International Workshop on Lobster Biology and Management, Sanriku, Japan, July 25-31, 1993, Abstract #233.
Size-specific shelter usage by juvenile American lobsters, Homarus americanus, under predation risk. Abstracts of the 20th Marine Benthic Ecology Meetings, Newport, RI, March 26-29, 1992.
Lavalli, K.L. and K. Brayer. Are responses to predators context sensitive? (in prep for Oikos Mini-Review).
Lavalli, K.L. and K. Brayer. Shelter usage by juvenile American lobsters, Homarus americanus, while under predation risk: the role of prior experience with predators. (in prep. for Animal Behaviour).
SKILLSLaboratory: automated sequencing, PCR, quantitative PCR, DNA isolation, genomic DNA isolation, plasmid isolation, DNA purification, in vivo mutation analysis, in vitro & in vivo DNA footprinting, spectrometry, fluorometry, radioisotope handling, isotopic labeling, allozyme/isozyme electrophoresis, polyacrylamide gel electrophoresis, agarose gel electrophoresis, aseptic techniques, bacterial cell culture, bacterial transformation, animal care (invertebrates, vertebrates, aquatic and terrestrial), fish husbandry, establishment and maintenance of experimental populations, plant care, reagent prep, phase contrast microscopy, SEM, hazardous waste/chemical management.
Field Research: SCUBA, small boat handling, data collection including use of suction samplers, current meters, quadrants, transect lines, CTD’s secci disks, seine nets, plankton nets, identification of marine plants, invertebrates, vertebrates.
Computer: Systems: PC and Macintosh
Programs: Microsoft Word (97), Excel (97), Access (97), PowerPoint (97) and Publisher (97). Adobe PhotoShop (4.0), CorelDraw. Systat, StatView, SuperANOVA, Molecular Analyst (1.5), Sequence Analysis, Sequence Navigator, Factura, Paradox, SlideWrite Plus (4.0).
Video & Photography: Video microscopy, underwater video, photomicrography, underwater photography, development of black & white prints and ectachrome slides.
General: Data analysis and manuscript/poster/conference presentation preparation.
ADDITIONAL WORK EXPERIENCE